高丹

2006年09月-2011年06月，北京化工大学与清华大学联合培养，化学工程与技术，博士

2002年09月-2006年06月，北京服装学院，应用化学专业，本科

2019年12月-至今，清华大学深圳国际研究生院，副研究员

2019年3月-2019年12月，清华大学深圳国际研究生院，讲师

2018年1月-2019年1月，斯坦福大学，访问学者

2013年8月-2019年3月，清华大学深圳研究生院，讲师

2011年7月-2013年6月，清华大学深圳研究生院，博士后

《Sensors》特邀客座主编；

《Frontiers in Pharmacology》特邀客座主编

文献检索与论文写作

生物制造实验

药物化学专题实验

研究方向围绕生物医药与健康领域，聚焦疾病早期诊断和新药研发技术瓶颈，以“微流控技术创新+人工智能赋能”为核心，开展新型微流控生物传感、高仿生器官芯片、以及器官芯片与AI融合赋能创新药研发三个方向的研究工作，致力于为疾病早期诊断提供精准手段、为新药研发注入智能化动力。作为项目负责人国家自然科学基金面上项目、青年基金，中国博士后基金、广东省自然科学基金项目、深圳市基础研究学科布局、基础研究自由探索等多个项目；参与“十二五”重大新药创制、973项目、国家863项目等多项。在Trends in Analytical Chemistry, Biosensors & Bioelectronics, Sensors and Actuators B: Chemical, Chemical Science, ACS Applied Materials & Interfaces, Analytical Chemistry, Lab on a Chip等学术期刊发表学术论文70余篇，申请专利7项，授权3项。

[1] Shi, J.; Teng, H.; Zhang, Z.; Liu, Y.; Gao, D.; Di, J.; Yang, Z.; Su, P.; Tan, Y.; Ma, J., Spatial microfluidic holographic integrated platform for label-free and high-dimensional analysis of cancer heterogeneity, Nature Communications 2025, 16, 5890.

[2] Zhang, X.; Chen, H.; Fan, T.; Zhu, X.; Tan, Y.*; Gao, D.*, A microfluidic biosensor based on multiple signal amplification for rapid and sensitive detection of E. coli O157:H7 and Staphylococcus aureus, Chinese Chemical Letters 2025, doi.org/10.1016/j.cclet.2025.111705.

[3] Peng, J.; Li, B.; Ma, Z.; Qiu, Z.; Hu, H.; Jiang, Y.; Gao, D.*, A microfluidic-based chemiluminescence biosensor for sensitive multiplex detection of exosomal microRNAs based on hybridization chain reaction, Talanta 2025, 281, 126838.

[4] Hu, J.; Gao, D.*, Recent Advances in Aptamer-Based Microfluidic Biosensors for the Isolation, Signal Amplification and Detection of Exosomes, Sensors 2025, 25, 848.

[5] Ma, Z.; Ma, M.; Cao, X.*; Jiang, Y.; Gao, D.*, Droplet digital molecular beacon-LAMP assay via pico-injection for ultrasensitive detection of pathogens, Microchim Acta 2024, 191, 430.

[6] Nan, X.; Lai, W.; Peng, J.; Wang, H.; Chen, B.; He, H.; Mo, Z.; Xia, Z.; Tan, N.; Liu, Z.; Wen, L.; Gao, D.; Chen, Q.*, In situ photoelectric biosensing based on ultranarrowband near-infrared plasmonic hot electron photodetection, Advanced Photonics 2024, 6, 026007.

[7] Ren, X.; Zhang, F.; Gao, G.; Gao, D.*; Su, P.*, Biophysical and Morphological Cell Features Retrieved by Digital Holographic Microscopy Correlate with Drug-Induced Changes in Cell Migration Behavior, Anal Chem 2024, 96, 20526-20534.

[8] Su, Z.; Hu, W.; Ye, L.; Gao, D.*; Lin, J.-M.*, An integrated microfluidic chip-mass spectrometry system for rapid antimicrobial resistance analysis of bacteria producing β-lactamases, Chinese Chemical Letters 2023, 34, 107790.

[9] Li, B.; Liu, X.; Gao, D.*; Ma, Z.; Peng, J.; Wang, X.*; Jiang, Y., A microfluidics chemiluminescence immunosensor based on orientation of antibody for HIV-1 p24 antigen detection, Sensors and Actuators B: Chemical 2023, 395, 134510.

[10] Chen, Y.; Gao, D.*; Zhu, Q.; Chu, B.; Peng, J.; Wang, J.; Liu, L.; Jiang, Y., Rapid exosome isolation and in situ multiplexed detection of exosomal surface proteins and microRNAs on microfluidic platform, Analyst 2023, 148, 2387-2394.

[11] Hu, W.; Wang, C.; Gao, D.*; Liang, Q.*, Toxicity of transition metal nanoparticles: A review of different experimental models in the gastrointestinal tract, J Appl Toxicol 2023, 43, 32-46.

[12] Gao, D.*; Ma, Z.; Jiang, Y., Recent advances in microfluidic devices for foodborne pathogens detection, Trends in Analytical Chemistry 2022, 157, 116788.

[13] Hu, W.; Gao, D.*; Su, Z.; Qian, R.; Wang, Y.; Liang, Q.*, A cellular chip-MS system for investigation of Lactobacillus rhamnosus GG and irinotecan synergistic effects on colorectal cancer, Chinese Chemical Letters 2022, 33, 2096-2100.

[14] Sun, D.; Fan, T.; Liu, F.; Wang, F.; Gao, D.*; Lin, J.M.*, A microfluidic chemiluminescence biosensor based on multiple signal amplification for rapid and sensitive detection of E.coli O157:H7, Biosens Bioelectron 2022, 212, 114390.

[15] Zhou, M.; Gao, D.*; Yang, Z.; Zhou, C.; Tan, Y.; Wang, W.*; Jiang, Y., Streaming-enhanced, chip-based biosensor with acoustically active, biomarker-functionalized micropillars: A case study of thrombin detection, Talanta 2021, 222, 121480.

[16] Li, J.; An, Z.; Sun, J.; Tan, C.*; Gao, D.*; Tan, Y.; Jiang, Y., Highly Selective Oxidation of Organic Sulfides by a Conjugated Polymer as the Photosensitizer for Singlet Oxygen Generation, ACS Appl Mater Interfaces 2020, 12, 35475-35481.

[17] Li, S.; Gao, D.*; Song, C.; Tan, C.*; Jiang, Y., Isotope Labeling Strategies for Acylcarnitines Profile in Biological Samples by Liquid Chromatography-Mass Spectrometry, Anal Chem 2019, 91, 1701-1705.

[18] Yuan, T.; Gao, D.*; Li, S.; Jiang, Y., Co-culture of tumor spheroids and monocytes in a collagen matrix-embedded microfluidic device to study the migration of breast cancer cells, Chinese Chemical Letters 2019, 30, 331-336.

[19] Song, C.; Gao, D.*; Yuan, T.; Chen, Y.; Liu, L.; Chen, X.; Jiang, Y., Microfluidic three-dimensional biomimetic tumor model for studying breast cancer cell migration and invasion in the presence of interstitial flow, Chinese Chemical Letters 2019, 30, 1038-1042.

[20] Gao, D.; Jin, F.; Lee, J.K.; Zare, R.N.*, Aqueous microdroplets containing only ketones or aldehydes undergo Dakin and Baeyer–Villiger reactions, Chemical Science 2019, 10, 10974-10978.

[21] Gao, D.; Jin, F.; Yan, X.; Zare, R.N.*, Selective Synthesis in Microdroplets of 2-Phenyl-2,3-dihydrophthalazine-1,4-dione from Phenyl Hydrazine with Phthalic Anhydride or Phthalic Acid, Chemistry-A European Journal 2019, 25, 1466-1471.

[1] Xinrui Zhang, Xueming Zhu, Jessica Hu, and Dan Gao, Chapter 2 Nucleic Acid Detection of Pathogenic Microorganisms on chip [M]/Tarafdar A, SirhiR, Kaur B P, et al. Microfluidics in Food Prcessing: Technologies and Applications. Boca Raton: CRC Press, 2025:00.10-54.

[2] Dan Gao, Chao Song and Jin-Ming Lin, Chapter 7 Microfuidics Mass Spectromety Combination Systems for single cell Analysis [M]/Jin-Ming Lin in Eds, Microfuidics for Single-Cell Analysis, Cham Springer, 2019. pp.163-196.

[1] 蒋宇扬，高丹，陈永丽，基于纳米给药系统筛选的三维实体瘤模型的微流控芯片及其制各方法和应用，专利号：ZL201710740198.0，授权日期：2019年11月12日。

[2] 蒋宇扬，高丹，孙冬丽，樊婷婷，微流控芯片、基于微流控芯片的检测系统及细菌的检测方法，专利号：ZL 202111582058.8，授权日期：2023年9月12日。

[3] 蒋宇扬，高丹，陈昱霖，同时检测外泌体内部microRNA和表面蛋白的试剂盒和方法，专利号：ZL 202111383188.9，授权日期：2024年9月27日。

清华大学优秀硕士学位论文指导教师

入选2024年全球前2%顶尖科学家榜单