[1] Shamul, J.G., Wang, Z., Gong, H., Ou, W., White, A.M., Garcia, D.P.M., Gu, S., Clyne, A.M., Quinones-Hinojosa, A., and He, X. Meta-analysis of the make-up and properties of in vitro models of the healthy and diseased blood-brain barrier. Nature Biomedical Engineering 2025; 9: 566-598

[2] Wang, Z., Stewart, S., Kumagai, M., Wang, E., Ou, W., Lu, Y., Meng, T., Xu, C., Hong, Y., Ren, S., and He, X. High-yield convenient mass production of high-quality homogenous human iPSC spheroids under Rock inhibitor-free 3D culture enabled by micropatterning and cold-triggered chemical-free cell detachment. Small Science 2025; 5: 2500201

[3] Mahanty, M., Ou, W., Zhu, X., Bromberg, J.S., He, X.*, and Rahaman, S.O.* A novel core-shell hydrogel 3D model for studying macrophage mechanosensing and foreign body giant cell formation. Advanced Healthcare Materials 2025; accept (*co-corresponding authors)

[4] Ou, W., Stewart, S., White, A.M., Kwizera, E.A., Xu, J., Shamul, J.G., Tkaczuk, K.H.R., Lu, X., and He, X. In-situ cryo-immune engineering of an immunologically hot tumor microenvironment with cold-responsive nanomaterials for cancer immunotherapy. Nature Communications 2023; 14: 392

[5] Stewart, S., Ou, W., Aranda-Espinoza, H., Rahaman, S.O., and He, X. Micromechanical characterizations and viscoelastic modeling reveal elastic and viscoelastic heterogeneities in ovarian tissue and the significant viscoelastic contribution to the apparent elastic modulus determined by AFM indentation. Acta Biomaterialia 2023; 168: 286-297

[6] Kwizera, E.A., Ou, W., Lee, S., Stewart, S., Shamul, J.G., Xu, J., Tait, N., Tkaczuk, K.H.R., and He, X. Greatly enhanced CTC culture enabled by capturing CTC heterogeneity using a PEGylated PDMS-titanium-gold electromicrofluidic device with glutathione-controlled gentle cell release. ACS Nano 2022; 16: 11374-11391

[7] Huang, H.*, He, X.*, and Yarmush, M.* Advanced technologies for the preservation of mammalian biospecimens. Nature Biomedical Engineering 2021; 5: 793-804 (*co-corresponding authors)

[8] White, A.M., Zhang, Y., Shamul, J.G., Xu, J., Kwizera, E.A., Jiang, B., and He, X. Deep learning-enabled label-free on-chip detection and selective extraction of cell aggregate-laden hydrogel microcapsules. Small 2021; 17: 2100491

[9] Jiang, B., Li, W., Stewart, S., Ou, W., Liu, B., Comizzoli, P., and He, X. Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells. Bioactive Materials 2021; 6: 4377-4388

[10] Xu, J., Liu, Y., Li, Y., Wang, H., Stewart, S., Van der Jeught, K., Agarwal, P., Zhang, Y., Liu, S., Zhao, G., Wan, J., Lu, X., and He, X. Precise targeting of POLR2A as a therapeutic strategy for human triple negative breast cancer. Nature Nanotechnology 2019; 14: 388-397

[11] Zhang, Y., Wang, H., Stewart, S., Jiang, B., Ou, W., Zhao, G., and He, X. Cold-responsive nanoparticle enables intracellular delivery and rapid release of trehalose for organic solvent-free cryopreservation. Nano Letters 2019; 19: 9051-9061

[12] Agarwal, P., Wang, H., Sun, M., Xu, J., Zhao, S., Liu, Z., Gooch, K.J., Zhao, Y., Lu, X., and He, X. Microfluidics-enabled bottom-up engineering of 3D microvascularized human tumor for drug discovery. ACS Nano 2017; 11: 6691-6702

[13] Zhao, S., Xu, Z, Wang, H., Reese, B., Gushchina, L., Jiang, M., Agarwal, P., Xu, J., Zhang, M., Shen, Y., Liu, Z., Weisleder, N., and He, X. Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction. Nature Communications 2016; 7: 13306

[14] Zhao, S., Zhang, L., Han, J., Chu, J., Wang, H., Chen, X., Wang, Y., Tun, N., Lu, L., Bai, X.-F., Martha, Y., Devine, S., He, X.*, and Yu, J.* Conformal nanoencapsulation of allogeneic T cells mitigates graft-versus-host disease and retains graft-versus-leukemia activity. ACS Nano 2016; 10: 6189-6200 (*co-corresponding authors)

[15] Choi, J.K., Agarwal, P., Huang, H., Zhao, S., and He, X. The crucial role of mechanical heterogeneity in regulating follicle development and ovulation with engineered ovarian microtissue. Biomaterials 2014; 35: 5122-5128