1. He, X., Li, W., and Jiang, B. System, device, and method for cell cryopreservation via sand-mediated ice seeding. US patent application (divisional) No. US19/263,995, filed on 07/09/2025

2. He, X. System, apparatus, and method for spheroidal pluripotent stem cell generation using cold-triggered detachment. US patent application No. US19/090,572, filed on 03/26/2025

3. He, X. and Ou, W. In-situ cryo-immune engineering (ICIE) for cancer immunotherapy. Disclosed to the University of Maryland Technology and Commercialization Office on 05/15/2022. U.S. provisional patent application (US63/480434) filed on 01/18/2023; US patent application No. US18/415,331, filed on 01/17/2024

4. He, X. and Jiang, B. Method for cardiac differentiation of human pluripotent stem cells. Disclosed to the University of Maryland Technology and Commercialization Office on 12/20/2020; US patent application No. US17/814,044, filed on 07/21/2022

5. He, X., Xu, J., and Shamul, J.G. Microencapsulation-based isolation of human pluripotent and multipotent stem cells and methods of making and using the same. Disclosed to the University of Maryland Technology and Commercialization Office on 04/17/2021. US patent application No. US17/663,372, filed on 05/13/2022

6. He, X. and White, A.M. System and method for selective microcapsule extraction. Disclosed to the University of Maryland Technology and Commercialization Office on 04/16/2021; US patent application No. US17/725,096, filed on 04/20/2022

7. He, X., Li, W., and Jiang, B. System, device, and method for cell cryopreservation via sand-mediated ice seeding. Disclosed to the University of Maryland Technology and Commercialization Office on 01/28/2021; US patent application No. US17/659,432, filed on 04/15/2022; USPTO patent No. US 12,382,953 (granted on 08/12/2025)

8. He, X. System, device, and method for single-cell encapsulation and culture. US provisional application (US63/013,173) filed on 04/21/2020. US patent Application No. 17/920440, filed on 04/21/2021. International patent application (PCT/US2021/028503) filed on 04/21/2022

9. He, X., Lu, X., Xu, J. pH-activated nanoparticles. US Provisional Application (US62/752,851) filed on 10/30/2018. International patent application (PCT/US2019/058907) filed on 10/30/2019. US patent application No. US17/289,425, filed on 10/30/2019. European patent application No. EP19880846.1, filed on 10/30/2019

10. He, X., Zhao, S., Weisleder, N., Liu, Z., and Xu, Z. Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction. Disclosed to OSU Technology and Commercialization Office on10/24/2013. International Application No. PCT/US2017/058837, filed on 10/27/2017

11. He, X., Agarwal, P., and Zhao, S. Microcapsules encapsulating living cells. USPTO patent application No. 14/075,912, filed 11/08/2013

12. He, X. and Zhang, W. Microcapsules for cell microencapsulation. USPTO patent application No. US20120231443, filed on 03/092012

13. He, X. Encapsulation and controlled release of small molecules for intracellular delivery using thermally responsive nanocapsules. US patent application (US12/705,07) filed on 02/12/2012; USPTO patent No. US 8,318,207 (granted on 11/27/2012)

14. He, X. Methods for augmentation of cell cryopreservation. US patent application No. US12/796,994, filed on 06/09/2010

15. He, X. Controlled drug delivery using a thermally responsive nanocapsule to augment cryoablation. US patent application No. US12/714,931, filed on 03/012010

16. He, X., Toth, T., Toner, M., and Ed, J. Methods for cryopreservation of cells. International patent application (WO/2007/120829A2). US patent application No. US12/226,300 filed on 04/12/2007). USPTO patent No. US9538745B2 (granted on 01/10/2017)